Phytochemical and antibacterial activities of newbouldia. Antimicrobial activity of extracts from fruits of aronia. Collection of plant material and extract preparation. The methanolic extract of caryophyllus aromaticus presented the highest antis. Antibacterial activity of plant extracts 247 brazilian journal of microbiology 2000 31.
Minimum inhibitory concentration mic assay were determined for these two extracts. Aloe vera gel is a bactericidal agent causing against pseudomonas aeruginosa and acemannan and cite this article. Evaluation of antimicrobial activity of some plant. Antibacterial activity of medicinal plant extracts article pdf available in brazilian journal of microbiology 384. Antibacterial and antifungal activities of plant part extracts against four pathogenic bacteria two grampositive and negative and three pathogenic fungi were investigated by the agar disk diffusion method. The antimicrobial effect of ethanol extracts of five plant extract against human bacteria namely streptococcus pyogenes atcc 19615, streptococcus. The search for new antimicrobial natural products from plant materials is essential in order to curb the menace of multiple antibiotics resistant pathogens. Antibacterial and antifungal activities from leaf extracts. However, ali mirzaei 29 showed that ethanolic extract of plant origanum vulgare shows significant antimicrobial activity.
However, the antimicrobial activity of several extracts of different plants was reported. The antimicrobial activity of the test organisms to the 11 plant extracts was screened by using the agarwell diffusion method perez et al. The antimicrobial activities of methanolic extracts of. Evaluation of antibacterial activity of these plant extracts was recorded in table 2 and illustrated in fig. Antimicrobial activity of selected medicinal plants against. The extract of medicinal plants were prepared using soxhlet apparatus for alcoholic extract, and water reflux for aqueous extracts. Antimicrobial activity of medicinal plant extracts and.
Absence of antimicrobial activity does not mean that the bioactive compounds are not present in the plant or the plant has no activity against microorganisms. Antibacterial and antifungal activities from leaf extracts of. Antibacterial activity of aqueous and ethanolic extracts of fresh and treated fruits, and fresh and treated extracts, expressed as inhibition zone diameter in millimeters. Studies of antimicrobial properties of different leaf. Essential oils extracted from five fiber varie ties of. The antimicrobial activity of plant extracts and phytochemicals was evaluated with antibiotic. Dec 25, 2001 secondly, the method used to assess antimicrobial activity, and the choice of test organisms, varies between publications janssen et al. Results and discussion antibacterial activity all three extracts of the plant tested showed varying d eg rof an t ib c lv sh species table 1. Pdf antibacterial activity of some plant extracts researchgate.
The high activity of the phenolic components may be further explained in terms of the alkyl substitution into the phenol nucleus, which is known to enhance the antimicrobial activity of. The search for new antimicrobial natural products from plant materials is. Antimicrobial activity of some plant extracts against. They are known to possess antioxidant, antiinflammatory, antibacterial, immunomodulatory and antisickling activities. Exactly 200 mg of each dried mass of leaf was added to 2 ml of solvents aqueous, chloroform and ethanol. Antimicrobial activity study of ethanolic extract of.
Apr 14, 2019 the plant extract treated fabrics were subjected to the antimicrobial activity test against the ecoli and s. The introduction of alkylation has been proposed to alter the distribution ratio between the aqueous and the nonaqueous phases. The extract of three medicinal plants used in nigerian folk medicine showed a highly antibacterial activity against some food borne pathogens. Evaluating antimicrobial activity of aloe vera plant extract.
Antibioticresistant microorganisms have been an evergrowing concern over the past years. Furthermore, chloroform and acetone extract from the leaves. Materials and methods plates were prepared for the antibacterial activity. The antibacterial activity of extracts of nine plant species with good. Antimicrobial activity of methanolic extract the antimicrobial activity of methanolic extract using disc diffusion method, p. Antimicrobial activity of selected medicinal plants. Antimicrobial tests for the leaves stem and roots of the studied plants were carried out against the pure cultures of and escherichia coli. Plant nametype of extract susceptible microorganism reference garcinia mangostana alpha. The extractions from latex are quit tricky in context with preparation of latex for the extraction purpose. Pdf antimicrobial activity of medicinal plant leaf extracts.
Thus, a varied range of natural products of plant, fungi and lichen origin were tested against two bacterial species, staphylococcus aureus atcc 25923 and escherichia coli atcc 25922, by two variants of the agar diffusion. The antimicrobial activity from ethanol and petroleum ether extracts against multiple microorganisms was re ported 8. Effect of plant extract on bacterial culture bacterial culture extra negative control. Extraction of plant latex is a complex emulsion of polymer micro particles in an aqueous medium.
Research article open access antibacterial activities of selected edible plants extracts against multidrugresistant gramnegative bacteria doriane e djeussi1, jaures ak noumedem1, jackson a seukep1, aime g fankam1, igor k voukeng1, simplice b tankeo1, antoine hl nkuete2 and victor kuete1 abstract. The residue the mixture was transferred into a percolator with overnight maceration. Introduction nature has been a source of medicinal agents since ancient times. Varying concentration of each extracts 200mgml, 150 mgml, 100mgml.
The fact that a plant extract exhibits antimicrobial activity is of interest, but this preliminary part of data should be trustworthy and allow researchers to compare results, avoiding work in which. Antimicrobial activity of polyphenols and alkaloids in middle. Antioxidant, antihypertensive, antihyperglycemic, and antimicrobial. The study has shown the presence of steroid, alkaloids and tannins. The plant extracts showed antibacterial activity but not antifungal activity against any of the fungi used. Antimicrobial activity of rosemary extracts rosmarinus officinalis l.
Antimicrobial activity of fruits of aronia melanocarpa and sorbus aucuparia 197 table 2. Five plant species were investigated to evaluate their antibacterial activity against food poisoning bacteria including two strains of gram positive bacteria b. The middle east encompasses a wide spectrum of plant diversity with over 20,000 different species in habitats ranging from deserts to snowcapped mountains. Antimicrobial activities of alcoholic extracts of neem leaves were used. Moringa oleifera is a tropical tree whose many economic applications and facility of propagation are arousing growing international interest. All in all, data about specific antibiotic properties of coumarins are scarce, although many reports give. Antibacterial and antifungal activities of plant part extracts against four pathogenic bacteria two grampositive and negative and three pathogenic fungi were investigated by the agar disk. Various parts of the plant such as the leaves, roots. Introduction even though pharmacological industries have produced a number of new antibiotics in the last three decades, resistance to these drugs by microorganisms has increased.
Medicinal plants, traditional uses, phytochemical effects, antimicrobial activity. In vitro antimicrobial activity of some medicinal plants. All in all, data about specific antibiotic properties of coumarins are scarce, although many reports give reason to believe that some utility may reside in these phytochemicals 26, 83, 193. Antimicrobial activity and phytochemical analysis of.
Antimicrobial activity of rosemary extracts rosmarinus. Bioactive aqueous extracts of twelve yucatecan plants. Antimicrobial activity of some medicinal plant extracts against bacteria causing diarrhoea by naomi tope komolafe submitted in accordance with the. Moreover, antimicrobial activity of different natural substances such as medicinal plant extract have been investigated against food borne bacteria. The significant activity shown by the extract of o. Box, 395, ethiopia abstract antibiotics provide the main basis for the therapy of microbial bacterial and fungal infections.
General antimicrobial activity was documented in woodruff galium odoratum extracts. The total glycosides, cardenolide glycosides, anthraquinone glycosides isolated from. Methods for in vitro evaluating antimicrobial activity. The antimicrobial activity of plant extracts and phytochemicals was evaluated with antibiotic susceptible and resistant microorganisms. Thus, a varied range of natural products of plant, fungi and lichen origin were tested. Our results revealed that chloroform, butanol, ethyl acetate extract of a. Antimicrobial activity of some medicinal plant extracts against bacteria causing diarrhoea by naomi tope komolafe submitted in accordance with the requirements for the degree of master of science in the subject life sciences at the university of south africa supervisor. Chemical composition, antifungal and anti aflatoxigenic activities of oils of ocimum species were investigated by some workers 21, 22. Antimicrobial activity of medicinal plants against human pathogenic bacteria 657 inflammatory properties of plants mahesh and satish, 2008. Pdf antimicrobial activity of medicinal plant leaf. The antibacterial activities of extracts were evaluated using the disk diffusion method as well as well diffusion method. This has led researchers to direct their attention onto plants to be able to discover new possible antimicrobial compounds. Antimicrobial activity of azadirachta indica neem against.
For example the crude methanolic extracts of neem plant have been shown to have strong antibacterial activity8. Pseudomonas showed resistance to all the solvents except to tulsi leaf ethanol extracts. The high activity of the phenolic components may be further explained in terms of the alkyl substitution into the phenol nucleus, which is known to enhance the antimicrobial activity of phenols pelczar et al. Phytochemical analysis of traditional medicinal plants and. Significant antimicrobial activity of plant extract had been observed. Organic solvent extraction ethanol and methanol extracts. Antimicrobial activity of crude extracts of three medicinal.
Evaluating antimicrobial activity of aloe vera plant extract in human life. Klebsiella the best antimicrobial activity was observed with eucalyptus leaf ethanol extracts. Antimicrobial activity of medicinal plant extracts and their. Antimicrobial activity of crude extracts of some plant leaves. Presence of inadequate quantities of active constituent or constituents in the extract to exhibit the antimicrobial activity can be the reason for the negative results. Extraction of plant latex is a complex emulsion of polymer micro particles in an aqueous. Pdf antibacterial activity of medicinal plant extracts.
It is in view of this, that the present research was set up to evaluate the antimicrobial activity of, using different. Evaluation of antibacterial activity of some medicinal plants extracts. The oil of the seeds, petroleum ether and methanol extracts of the whole plant of cannabis sativa belonging to the family cannabinaceae were screened for their antimicrobial activity against. Secondly, the method used to assess antimicrobial activity, and the choice of test organisms, varies between publications janssen et al.
Antimicrobial activity of essential oils and other plant. Antimicrobial activity of medicinal plants against human. Evaluation of antimicrobial activity of different solvent. Antimicrobial activity and phytochemical screening of stem. Pdf antibacterial activity of medicinal plant extracts researchgate.
Research article open access antibacterial activities of. Antimicrobial activity of polyphenols and alkaloids in. Acetone technical grade, merck was used as an extractant in the assays using a ratio of 1. The aim of this work was to evaluate the antimicrobial activity of synthesized heterocyclic compound shcs like as furan derivatefd 1furan2ylthioxopropenon, thiophene derivate thd 2cyano2scthiophene2yl1,2dithiol3ylidene. Studies of antimicrobial properties of different leaf extract. Some promising plants having antimicrobial activity against multidrug resistant strains. The fact that a plant extract exhibits antimicrobial activity is of interest, but this preliminary part of data should be trustworthy and allow researchers to compare results, avoiding work in which researchers use the antimicrobial activity investigation only as a complement to a phytochemical study.
Evaluating antimicrobial activity of aloe vera plant. Some bacteria showing resistance to certain antibiotics were sensitive. Antimicrobial activity and phytochemical analysis of calotropis gigantea root, latex extracts. A method frequently used to screen plant extracts for antimicrobial activity is the agar disc diffusion technique morris et al. The dried extract was kept at 4 c for antibacterial activity assay. Bacteria, antimicrobial activity, plant extracts, solvents. Evaluation of antimicrobial activity of some plant extracts. Control experiments comprising inoculums without plant extract. Antimicrobial activity of medicinal plant extracts and their synergistic effect on some selected pathogens desalegn amenu college of natural and computational science, biology. Introduction medicinal plants are abundant source of antimicrobial molecules. Antimicrobial activity in leaf extract of neem azadirachta indica against human pathogenic bacteria. Studies on some plant extracts for their antimicrobial. Antimicrobial activity of medicinal plant extracts and their synergistic effect on some selected pathogens desalegn amenu college of natural and computational science, biology department, wollega university, p. Antibacterial activity of plants extracts against pathogenic bacteria.
The antibacterial effect of some medicinal plant extracts. Azadirachta indica leaves and barks were collected from. Evaluation of antibacterial activity of some medicinal. The plant extract treated fabrics were subjected to the antimicrobial activity test against the ecoli and s. Nutrient agar 20ml was dispensed into sterile universal bottles. Furthermore, chloroform and acetone extract from the leaves of anthocephalous cadamba had been reported to have prominent antimicrobial activity against several gram positive and gram negative human pathogenic bacteria 30. Antimicrobial activity of some plant extracts against bacterial strains. Water extract of garlic and clove possesses antimicrobial activity. Determination of in vitro antimicrobial activity of five sri. In addition, the possible synergistic effects when associated with antibiotics were. Antimicrobial finish on textiles using plant extracts.
An inoculum suspension was swabbed uniformly to solidified 20 ml muellerhinton agar mha for bacteria and sabouraud dextrose agar sda for fungi and the. Antimicrobial, antioxidant activity and phytochemical. For example the crude methanolic extracts of neem plant have been shown to have strong. Determination of antimicrobial activity the agar well diffusion method described by pelczar et al. Medicinal plants constitute an effective source of antimicrobial natural products. Antibacterial activity of five medicinal plant extracts. May 15, 2019 antibioticresistant microorganisms have been an evergrowing concern over the past years. Antimicrobial, antioxidant activity ijpcr, june 2016, volume 8, issue 6 page 586 table 2. This has led researchers to direct their attention onto plants to be able to discover. Data on antimicrobial activity measure as a zone of inhibition of aqueous extracts of eleven plant species and two antibiotics on.
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